EGFR anticorps (pTyr869)

N° du produit ABIN126761

L’anticorps Souris Monoclonal anti-EGFR a été validé pour WB, IF, IHC (p), IP, EIA et IHC (fro). Il convient pour détecter EGFR dans des échantillons de Humain et Souris.

Aperçu rapide pour EGFR anticorps (pTyr869) (ABIN126761)

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EGFR est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 12A3

Détail du produit anti-EGFR anticorps

Épitope: pTyr869

Specificité: Clone 12A3 specifically recognizes EGFR phosphorylated at Tyrosine 869 and detects EGFR activation after interaction with src kinases. Antibody AM00031PU-N does not crossreact with the highly homologues pTyr 877 of activated erbB2.

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse.

Attributs du produit: Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1

Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

Ingrédients: incl. positive Control

Immunogène: Phosphopeptide conjugated to KLHEpitope: Phosphotyrosine 869AA Sequence: E K E pY H A E

Isotype: IgG1

Information d'application

Indications d'application: ELISA: Use at 0.1 μg/mL (direct ELISA). Immunocytochemistry: Use at 1-10 μg/mL. Immunohistochemistry on Frozen and Paraffin Embedded Material. Immunoprecipitation: Use at 1-10 μg per 10^6 vanadate-treated A431 cells. Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer (cat#AS00002BU-N). Included Positive Control: Cell lysate delivered with this product (See Protocol below! ).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Positive control cell lysate provided: HepG2 EGF Treated - recommended for immunoblottingFormulation: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15 min. withEGF. Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGEsample buffer, mix and incubate at 90°C for 5 min. Store in aliquots at -20°C. Avoidrepeated freezing and thawing. Applications: 20 µl of positive cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (mini gel) forHRPO/ECL detection of the target proteins.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in 1 mL distilled water for 15 min at RT.

Buffer: PBS containing 0.09 % Sodium Azide / PEG and Sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution,aliquote and freeze in liquid nitrogen, reconstituted antibody can be stored frozen at-80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3months. Avoid repeated freeze / thaw cycles!

Date de péremption: 12 months

Détails sur EGFR

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Autre désignation: EGFR / ERBB1

Sujet: EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1

ID gène: 1956

UniProt: P00533

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins